Journal article
A modular BAM complex in the outer membrane of the α-proteobacterium caulobacter crescentus
K Anwari, S Poggio, A Perry, X Gatsos, SH Ramarathinam, NA Williamson, N Noinaj, S Buchanan, K Gabriel, AW Purcell, C Jacobs-Wagner, T Lithgow
Plos One | PUBLIC LIBRARY SCIENCE | Published : 2010
Abstract
Mitochondria are organelles derived from an intracellular α-proteobacterium. The biogenesis of mitochondria relies on the assembly of β-barrel proteins into the mitochondrial outer membrane, a process inherited from the bacterial ancestor. Caulobacter crescentus is an α-proteobacterium, and the BAM (β-barrel assembly machinery) complex was purified and characterized from this model organism. Like the mitochondrial sorting and assembly machinery complex, we find the BAM complex to be modular in nature. A ∼150 kDa core BAM complex containing BamA, BamB, BamD, and BamE associates with additional modules in the outer membrane. One of these modules, Pal, is a lipoprotein that provides a means for..
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Awarded by National Institute of General Medical Sciences
Funding Acknowledgements
Work in the authors' laboratories is supported by grants from the National Health and Medical Research Council (508911; to AWP and TL), and an ARC Linkage Infrastructure Equipment and Facilities grant (LE0882913). KA is supported by a Monash University Research Scholarship; AP is supported by a National Health and Medical Research Council Training Fellowship; SP is supported by a Pew Latin American Fellowship. SB and NN are supported by the Intramural Research Program of the National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases. The CJW laboratory is supported by the National Institutes of Health (GM065835 and GM076698). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.